The content of the system of departmental control of quality and safety of the medical activity organized and realized by Department of health care of the city of Moscow in relation to the subordinated state medical organizations, along with the state and internal control is considered.

Objective. Study analysis on the concentration of the laboratory markers in patients with cardiovascular disease (CVD) before percutaneous coronary intervention (PCI) and within a year after coronary stent introduction. Material and methods. This article presents the data collected through annual (one-year) observation of 71 patients after they had undergone PCI (58 patients, who didn’t have any complications, and 13, who got restenosis or clinical significant stenosis in a new site). All participants of the study had their investigated clinical blood test with automatic counting of the leukocyte formula, C-reactive protein, fibrinogen, uric acid, markers of lipid metabolism, calculating the coefficient of atherogenicity, the concentration of secretory phospholipase A2 and the level of antibodies to oxidized low-density lipoproteins, before the operation as well as 3, 6 and 12 months after PCI. Results. Significant differences in the level of peripheral biomarkers in groups with different variants of postoperative course were not revealed. A greater variability in the values of inflammation markers throughout the observation period and an increase in the level of secretory phospholipase A2 at 6 months after percutaneous coronary interventions in the group followed by complications of stenting (p < 0.05) is shown. Patients with adverse course of the postoperative period are characterized by a lower level of antibodies to oxidized LDL, with a lower coefficient of variation, compared to the control group. Conclusions. Determination of the activity of secretory phospholipase A2 (IIA) in the serum after PCI can be useful as a prognostic marker for identifying patients at increased risk of adverse coronary events.

The brief report provides information on the pathophysiology of steroid hormones cortisol and dehydroepiandrosterone sulfate. Their role in the pathogenesis of a number of somatic diseases and depression is shown. The data on the determination of the ratio of these hormonal parameters and the laboratory assessment of the hormonal balance of the body are discussed.

The article describes a new approach to the assessment of hemolysis index (HI) on the example of two tests - alanine aminotransferase (ALT) and aspartate aminotransferase (AST), measured in blood serum samples on an automatic biochemical analyzer cobas 6000. This approach is based on the calculation of the Reference Change Value (RCV) taking into account the reference intervals for ALT and AST. This allows you to define a statistically significant the significance of differences between the two laboratory results from one patient and the deletion of obtaining false positive results.

Patients with severe sepsis have multiple dysfunction of coagulation system [1]. The routine coagulation tests provide possibility to estimate plasma part of system of coagulation, but without estimation of specific cellular part of this system. Appearance of new global coagulation tests provides assessment of physical characteristics of the clot and kinetics of it formation, so as fibrinolysis and retraction of clot. The clinical study of coagulation system has been conducted in order to estimate the roll of the thrombocytic fraction, which causes the disturbance of coagulation in patients with sepsis and septic shock. The patients have been divided into two groups: Group 1 consisted of 26 patients without thrombocytopenia. As Group 2 we enrolled 18 patients with this disturbance. The routine coagulation tests (prothrombin index Quick s value, activated partial thromboplastin time (aPTT), fibrinogen testing included), platelet count and assessment of platelet’s morphology and thromboelastography has been completed in every patient. Results: parameters of routine coagulation tests show no dependence of platelet count. Also, during the estimation of parameters of thromboelastogram has been noticed that patients with thrombocytopenia had increased clot reaction time (R), clot kinetics (K, time from clot formation to time amplitude reaches 20 mm), decreased value of angle a and maximum amplitude (MA). Based on data obtained during study, may be assumed that for patients with sepsis and septic shock are characteristic deficit of the coagulation proteins are the core components of the coagulation system and activation of cellular part of coagulation system. Developing of thrombocytopenia does not lead to manifest hypocoagulation. Using both routine coagulation tests and thromboelastinography in interpretation of results gives us better insight of complex assessment of system of coagulation and provides guideline in treatment of those patients.

We discussed data about biological properties of human platelets, not containing or containing very small granules' volume. There are few types of granule-devoted platelets with different structural features, which may be relevant for fundamental and applied research.

In diabetes mellitus and a number of other diseases, an excessive accumulation of β-ketone is observed, which is not detected in the urine by the standard method. Determination of the concentration of β-ketone in the blood is necessary for diagnosis and monitoring of the treatment under ketosis. Evaluation of the patient s condition with this indicator helps to more efficient and quick removal of the patient from the pathological condition. The availability of the study is provided by the availability of sets of reagents and devices for the rapid determination of the analyte.

A study was made of the main metabolites of ferrokinetics (hepcidin 25, interleukin 6, soluble transferrin receptors, ferritin, erythropoietin) for the differential diagnosis of iron deficiency syndromes (AIN). 62 patients with colorectal cancer were examined before treatment: 35 men and 27 women. Age from 31 to 89 (61.5 ± 1.7) years. Three types of anemia are distinguished. 21 people - with iron deficiency (IDA), 23 people with iron deficiency erythropoiesis (ZHDE) and with functional iron deficiency (FJD) - 18 people. Microcytosis and hypohromia of erythrocytes were characteristic for all types of anemia. The concentration of RF, HP25, IL-6 was reduced in patients with IDA and significantly increased in the group with PDJ in comparison with the control. In contrast to IDA, a high level of RF was noted in patients with ZHDE. The indicators of rRTF exceeded the upper limit of the norm in all 3 groups. EPO analysis showed that lower production was observed in all (100 %) patients with FJD, and therefore in patients with a common tumor process (stage III-IV), to a lesser extent in patients with IDA and ZHDE. Further investigation of the expression of GP25, IL-6 is especially important in the differential diagnosis of AZN in combination with IDA and PDJ, since the treatment is fundamentally different. The prospect is a pathogenetic approach to the treatment of AZN in cancer patients using anti-hepatic drugs based on antibodies to the hormone, inhibitors and blockers of its expression under the control of the concentration of GP25 and, possibly, IL-6.

Over the past decades, has been recorded a steady increase of patients with heart failure with preserved ejection fraction (HFpEF), which is a complex clinical syndrome and includes a combination of several concomitant diseases. Based on the results of some studies, it is suggested that at the base of HFpEF there are alterations of the arteriolar-capillary bed. In our research, we’ve studied the microcirculation disorders in patients with HFpEF and the concentration of serotonin in the blood plasma as a possible biomarker for assessing of systemic microcirculation.

The study of the effect of the liposomal form of α-lipoic acid on the human blood coagulation system. There was no significant effect of phosphatidylcholine liposomes containing and not containing а-lipoic acid on the plasma link of haemostasis. it is shown that the liposomal form of α-lipoic acid at concentrations of 0.2-0.4 mg/ml dose-dependent inhibits platelet aggregation in the platelet-rich human blood plasma induced by arachidonic acid, and prevents the reduction of platelet granularity.

Aim of research. To identify the features of the course of cryptosporidiosis, to improve methods of laboratory diagnosis and treatment. Patients and methods: 266 patients of the infectious hospital were observed. To find out oocysts of Cryptosporidia were used: classical microscopic method of modified coloration of fecal swabs according to Tsiol-Nielsenus with the following study in immersion microscopy; the qualitative determination of antigens by the RINA QUICK Cryptosporidium pavrum immunochromatographic test; Immunological analysis (Cryptosporidium Antigen (Stool) ELISA). The intestinal microfiora was studied for dysbacteriosis (Standard for managing patients with intestinal dysbacteriosis (N. M. Gracheva et al., 2004). In complex treatment of 33 patients was used sporobacterin 2 ml twice a day for 10 days. Results. In 26.3 % of patients with infectious diseases, cryptosporidia was found. In 94.3 % of cases, the latent flow was diagnosed, and in 5.7% the intestinal form. In acute period all patients had dysbacteriosis of the intestine of 2-3 degrees. After treatment, there was a positive dynamics in the microbiocenosis of the intestine, which manifested as an increase in the number of patients with minor disturbances in the microfiora (DI 1 25.7 %). Conclusion. When 266 patients were examined, in 26.3 % of cases, oocysts cryptosporidia were found. In the clinical picture, intestinal forms with a latent course dominated against the background of pronounced dysbiotic disorders (DI2-DI3). The inclusion of sporobakterin in the complex therapy of patients had a positive clinical and microbiological effect.