The review considers mobile health technologies (mHealth) - a new, rapidly developing field of medicine. Extremely promising are hybrid devices based on microfluidic technologies, mobile devices for monitoring diabetes mellitus, the use of mobile phones in immunoassay, microscopy in the diagnosis of cancer. Amplification technologies of mobile health care are used, in particular, in the diagnosis of infectious and parasitic diseases. All conditions for the emergence of mass open online medicine (IOM) have been created.

Achievements in the treatment of renal cancer are associated with antiangiogenic therapy. Therefore, exploration of molecular markers characterizing its angiogenic activity are on the agenda. Objective of the study: comparative evaluation of VEGF, VEGFR 1 and VEGFR2 content in blood serum of healthy persons, renal cancer and benign tumor patients, analysis of associations with the main clinical and pathologic features. 99 cancer and 10 benign renal tumor patients comprised the study group. Control group involved 80 persons. Concentration of the proteins studied was measured with direct ELISA kits (Quantikine®, R&D Systems, USA). VEGF, VEGFR 1 and VEGFR2 levels in the sera of renal cancer patients were significantly higher than in control group. Serum VEGF was also higher in renal cancer patients than in those with benign tumors. At 365 pg/m cut-off level VEGF diagnostic sensitivity for primary renal cancer comprised 67 %o, with specificity 70 %. Only serum VEGFR 1 level was positively associated with clinical stage, T and N indices. No associations with tumor histological type or differentiation grade were found. Conclusion: VEGF and its receptors' levels in blood serum have limited diagnostic value in renal cancer, but might be regarded as promising markers for monitoring of antiangiogenic therapy results and prediction of its efficiency.

The diagnosis of acute myeloid leukemia (AML) was examined in the article, and expression on blast cells of AML of linearly non-resolved and pan-myeloid antigens was analyzed using the eight-color Euro-Flow flow-cytometric protocols. In the analysis of primary immunophenotypes of blast cells, 36 patients with AML have a number of immunophenotypic features of blasts in various FAB variants of acute myeloid leukemias. A greater percentage of blast cells with the expression of myeloperoxidase, HLA-DR molecule and CD117 receptor c-kit were detected in the granulocyte leukemia group, while promyelocytic leukemia showed CD123⁺⁺-phenotype and was characterized by a more pronounced percentage of CD13+-blasts. The data obtained can be used to develop cytometric protocols for diagnostic immunophenotyping and to identify parallels with cytogenetic aberrations of the blast cell in AML. In addition, the individual immunophenotypic profile of blast cells revealed in the primary diagnosis on the basis of a detailed immunological study using Euro-Flow panels can be used for subsequent monitoring of the minimal residual disease in the case of AML.

In the article, the results of mitochondrial DNA sequencing in the group of unrelated patients with mitochondrial encephalo-myopathy diagnosed are presented. Significance of individual genetic particularities is evaluated through example of two patients with different ethnical background. In spite of not revealing confirmed pathogenic variants, we found several individual particularities of mitochondrial DNA, which may have clinical significance, in each patient. Through these examples modern approaches of personalized diagnostics are considered. In addition, robust relationship between laboratory and post-laboratory methodics in molecular genetic analysis are demonstrated.

Methods of laboratory diagnostics play a leading role in the etiological diagnosis, determining etiology, determining the severity and prognosis of acute pancreatitis. The study presents an analysis of the capabilities of modern methods of laboratory diagnostics in clinical practice, in accordance with the principles of evidence-based medicine, demonstrated the relationship of their capabilities, strategy and consistency in the application, their performance in each specific clinical situation.

Interpretation of laboratory results is one of the most difficult tasks in the work of a doctor. Consideration of errors affecting the results of the analysis and their use in the evaluation of results is necessary for the doctors of different specialties. To interpret the results it is important to understand the accepted terminology norm, reference values and the gray zone '. The paper presents main principles of evaluation of the results obtained in different laboratories, different measurement units, different methods in relative and absolute terms of results, it is important for the doctors of clinical laboratory diagnostics and the clinicians.

The human platelet preparing for different aims of regenerative medicine was described in the light of upgrading methods, based on platelets’ biological potential stimulation and realization.

In acute ethanol intoxication in erythrocytes of patients oxidative modification of proteins is enhanced and glutathione content is reduced, in blood plasma observed a significant decrease in the content of transferrin and ceruloplasmin. Glutathion and proteins antioxidants ceruloplasmin and transferrin are targets for the action of ethanol.

The article describes practicable elements for comprehensive on-going assessment of referral laboratory performance to fulfill ISO 15189 requirements. Specific plan and report examples can be used as baseline guidelines to establish, document and implement laboratory's individual procedures in accordance with laboratory's in-house demands.

The article considers the main problems in the organization of epidemiological surveillance of infectious diseases and suggests ways to address them.