The assessments of the prevalence of emergencies among medical workers of a multiprofile hospital of Vologda were undertaken. It is established that the most frequent medical emergencies in the institution arise among surgeons, anesthesiologists-resuscitators, nurses performing invasive manipulations. The main risk factors for emergencies are surgical interventions, injections and blood sampling, catheterization of the patient s vessels.

The interactions of different strains of Lactococcus lactis ssp. lactis were studied. lactis and pathogens of hospital infections of different etiology. The selection of the most effective strains of lactic bacteria isolated from cow s milk, national products of therapeutic and prophylactic use of different territorial zones of Russia and obtained by cellular engineering. Study of the antimicrobial spectrum of the action of Lactococus lactis ssp. lactis of different origin showed that natural K-205 strains isolated from Kurungi and 194, isolated from Buryatia’s milk, as well as recombinant F-116, F-119 possess a broad spectrum of antibacterial and fungicidal action against infectious agents. The most antagonistic activity to Klebsiella pneumoniae (clinical isolates, the causative agent of hospital infections) is strains 194, K-205 and acid-forming strain 729. It has been established that strains of K. pneumoniae are multidrug-resistant, and in L. lactis subsp. lactis susceptibility to antibiotics is strain-specific. The most effective strains of L. lactis subsp. lactis can be recommended for the use of strains as probiotic cultures in the postoperative period, which will allow them to be used for complex treatment in combination with antibiotic therapy.

In present review the groups of streptococci which are associated with human diseases were considered. Their clinical role is not sufficiently recognized by the medical community. Modern approaches to phenotypic identification of Streptococcus species were discussed. The possible ways of overcoming the difficulties of routine laboratory connected with microbiological diagnosis and antibiotic treatment of streptococcal infections were also discussed.

A Russian test kit for detecting IgG-antibodies to individual antigens of Herpes Simplex Viruses (type 1 and type 2) by immunoblotting (Western-Line-Blot) was designed. The new test kit is intended for confirmation of positive screening results and for differentiation of HSV-1 and HSV-2 infections. It has sensitivity and specificity not conceding one of its analogues, the Anti-HSV-1/HSV-2-gG2 EUROLINE-WB (IgG) (Euroimmun AG, Germany). Meanwhile, it is characterized by a number of consumer characteristics that ensure more efficient use of the kit.

The article presents a current approach л. И. Железова to study the character of large intestine microbiota in case of rotavirus diarrhea in children aged from 2 months to 18 years, who were treated in the period of years 2015-2017 in Pediatric Research and Clinical Centre for Infectious Diseases (Saint Petersburg, Russia), including clinical methods like data collection of clinical and anamnestic information about the patient, analysis of the character of clinical manifestations, sampling of fecal matter for viruses, pathogenic and conditionally pathogenic microorganisms and disbacteriosis, and also selection of paired blood serum samples for RA, reaction of agglutination with autostrains, and laboratory investigations like primary stool culture for acute intestinal infection agents, isolation and identification of etiologically significant strains by mass-spectrometer Bruker (DaltonikMALDI Biotyper, Germany) with identification of antibioticgrams and studying the genetic and phenotypic features of microorganisms, analysis of the contents of large intestine for disbacteriosis, PCR to reveal virus DNA-RNA, study of paired blood serum samples in the dynamics of the disease according to RA with autostrains and identification of the level of local nonspecific secretory A (SIgA) immunoglobulin in stool filtrates. Established the leading role of RVI in the structure of viral diarrhea in children identified age-related features of RV diarrhea as well as the status of the microbiota of the colon with mono-RVI, RVI, associated with virus and RVI, associated with a bacterial process, correlations were found between the severity of RVI in children, the degree of disorder of the microbiota, level D and defined genophenotypic characteristics of microorganisms. The results obtained define the tactics of treatment of children suffering from RVI focuses not only on effective causal and symptomatic therapy, but also for timely recovery of the intestinal microbiota and the level of non-specific secretory immunoglobulin A.

The analysis of epidemiological situation on HIV and HIV-associated infections with viral hepatitis C and lung tuberculosis during the 2012-2016 years was performed. The increase of prevalence of co-infection in Volgograd region is found.

The processing of endoscopes is one of the very difficultto-solve tasks due to their complexity and study issues in different countries. This is attributable to the complexity of their design, susceptibility to external influences, and the need to quickly perform this manipulation. The application of current approaches can ensure the safety of patients, the preservation of endoscopes, and the rapidity of their processing.

Relevance. Lichen planus is a subacute or chronic disease, affecting skin and oral mucous membranes. There is no accurate data oа the incidence of lichen planus is not, due to access of patients not only to dentists, but also to the dermatologists. There are typical reticular clinical form of oral lichen planus, and 6 atypical clinical: hypertrophic, exudative-hyperemic, bullous, erosive, atrophic and atypical. However, only reticular form is asymptomatic, while others are accompanied with dryness, pain and burning, and erosive form of lichen planus has statistically significant malignant potential. Purpose. The purpose of this study was to investigate the frequency of oral lichen planus in the general structure of lichen planus of the skin. Material and methods. The present study was based on screening of 338 patients with lichen planus - residents of the Moscow region. In thise group of patients were studied clinical features of different forms of lichen planus of skin and mucous membranes, especially their course and frequency of associations of skin lichen planus with oral lesions. Results. According to the study, in patients with typical lichen planus skin lesion reticular form of oral lichen planus was presented in 102 (of 30.18 %) cases and atypical forms were diagnosed in 26 (7,69%) cases. Atypical lichen planus of the skin, diagnosed in 81 patients, in 41 (50,6 %) cases was associated with oral lesions: in 12 (29,3 %) cases with reticular form, and in 29 (70,7%) with atypical forms. In 16 cases the process was isolated, localized in oral cavity and in each case it was with atypical forms. Conclusion. Oral lichen planus was diagnosed in 169 (50 %) patients with lichen planus of the skin, and with about the same (of 37.8 35.8 %) frequency it was associated with typical and atypical forms of skin lesions. The most severe and resistant to therapy was erosive oral lichen planus of the mouth, especially in vulvovaginal-gingival syndrome.