Morphofunctional state of pancreas in alloxan-induced diabetes mellitus

Abstract

The work was carried out on experimental material. 75 white male rats weighing 250-300 grams were injected a solution of alloxane tetrahydrate intraperitoneally at a dose of 150 and 250 mg per 1 kg of body weight. Before the administration of alloxan, the concentration of glucose in the blood was determined. The rats were withdrawn from the experiment on the 7th, 14th, 21st, 28th and 35th day. Piece of pancreatic tissue was taken for histological examination. Pieces were fixed in 10 % buffered formalin, conducted through alcohols of increasing strength and poured into paraffin. From the paraffin blocks, sections of 5-6 microns thick were prepared. The sections were stained with hematoxylin and eosin, picrofuxine according to Van Gison, toluidine blue, according to Mallory. With the introduction of alloxan in laboratory animals develops diabetes. The glucose level in the blood rises on the first day to 14.7 ± 0.03 mmol/l (iin the control 4.8 ± 0.03 mmol/l) and gradually decreases on the 21st day to 6.0 ± 0.03 mmol/l. There is a significant decrease in the body weight of laboratory animals. When 150 mg of alloxan is administered, moderate diabetes develops, with the introduction of 250 mg, severe diabetes develops. Histological examination revealed a decrease in the number and size of Langengharx islets, dystrophic changes, pycnosis and necrosis of B-cells, devastation of islets. The number of B-cells decreases to 25 % (control 75 %).