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Possibilities of flow cytometry, PCR and sequencing in early diagnosis of atherosclerosis activity
https://doi.org/10.33667/2078-5631-2024-4-7-11
Abstract
Atherosclerosis (AS) is a multifactorial disease leading to increased cardiovascular risks. Currently, the diagnosis of AS is limited to imaging methods of atherosclerotic plaque assessment and laboratory methods of dyslipidemia diagnosis, which do not provide an understanding of the activity of processes occurring in the atherosclerotic plaque. The aim of this review: to generate potential markers of AS activity. Macrophages play an important role in atherogenesis. Macrophages have several phenotypes, determining which can be concluded about the activity of the inflammatory process in the plaque. Genetic markers, such as microRNAs, circular RNAs and factors regulating their expression, play an important role in the development of atherosclerosis. Methods of flow cytometry, PCR and sequencing are promising for studying these markers. Markers of atherogenesis activity are essential in diagnosis, as well as in the choice of personalized treatment of patients.
About the Authors
A. P. Roytman
Russian Medical Academy of Continuous Professional Education
Russian Federation
Russian Federation
Roitman Alexander P., DM Sci (habil.), professor at Dept of Clinical Laboratory Diagnostics with a course in Laboratory Immunology
Moscow
N. S. Markina
Russian Medical Academy of Continuous Professional Education
Russian Federation
Russian Federation
Markina Nadezhda S., resident of Dept of Clinical Laboratory Diagnostics with a course in laboratory immunology
Moscow
V. V. Dolgov
Russian Medical Academy of Continuous Professional Education
Russian Federation
Russian Federation
Dolgov Vladimir V., Doctor of Medical Sciences, professor at Dept of Clinical Laboratory Diagnostics with a course in Laboratory Immunology
Moscow
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Review
For citations:
Roytman A.P., Markina N.S., Dolgov V.V. Possibilities of flow cytometry, PCR and sequencing in early diagnosis of atherosclerosis activity. Medical alphabet. 2024;(4):7-11. (In Russ.) https://doi.org/10.33667/2078-5631-2024-4-7-11
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