Seroconversion kit of control cats sera against antigen CAG1 of Toxoplasma gondii
Abstract
The aim of this work was to obtain seroconversion panel of control sera to the CAG1 antigen of T. gondii and evaluation of clinical information value of laboratory methods and corresponding kits of reagents. Seronegative for T. gondii 3 cats were immunized with recombinant SAG1 antigen in combination with adjuvants. The venous blood samples obtained on days 6-26 were studied in enzyme immunoassay (ELISA) and the reaction indirect immunofluorescence (RIF). Results RIF and ELISA with different sets of reagents were comparable, and more high analytical sensitivity of the RIF was shown. To create seroconversion panels of blood serum it is necessary to keep in mind the individual characteristics of the immunological reactivity of experimental animals, and also the features of used methods of immunization.
About the Authors
S. G. Mardanly
State University of Humanities and Technology; ECOlab Co
Russian Federation
Russian Federation
N. V. Bahilina
State University of Humanities and Technology
Russian Federation
Russian Federation
E. A. Amelina
ECOlab Co
Russian Federation
Russian Federation
N. V. Smirnova
ECOlab Co
Russian Federation
Russian Federation
P. .. Miethe
Forschungszentrum fur Medizintechnuik und Biotechnologie GmbH
Russian Federation
Russian Federation
D. .. Barnewitz
Forschungszentrum fur Medizintechnuik und Biotechnologie GmbH
Russian Federation
Russian Federation
B. .. Bruzera
Forschungszentrum fur Medizintechnuik und Biotechnologie GmbH
Russian Federation
Russian Federation
E. .. Willhauk
T & В Innovationen GmbH
Russian Federation
Russian Federation
S. V. Rotanov
ECOlab Co
Russian Federation
Russian Federation
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Review
For citations:
Mardanly S.G., Bahilina N.V., Amelina E.A., Smirnova N.V., Miethe P..., Barnewitz D..., Bruzera B..., Willhauk E..., Rotanov S.V. Seroconversion kit of control cats sera against antigen CAG1 of Toxoplasma gondii. Medical alphabet. 2017;3(30):43-47. (In Russ.)
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