EDTA‑dependent pseudothrombocytopenia in child (clinical case report)
https://doi.org/10.33667/2078-5631-2021-13-51-54
Abstract
Ethylenediaminetetraacetic acid (EDTA)-dependent pseudothrombocytopenia is the phenomenon of a spurious low platelet count due to antiplatelet antibodies that cause platelet clumping in blood anticoagulated with EDTA. The aggregation of platelets in EDTA-dependent pseudothrombocytopenia is usually prevented by other anticoagulants, such as sodium citrate. EDTA-dependent pseudothrombocytopenia has never been associated with hemorrhagic diathesis or platelet dysfunction. In this article, a 2,5-year-old boy with EDTA-dependent pseudothrombocytopenia is presented because of rare presentation. We report that EDTA can induce platelet clumping, and thus spuriously low platelet counts. However, aggregation of platelets was not detected in blood samples with sodium citrate, and platelet count was normal.
About the Authors
N. A. Sokolova
Russian National Research Medical University n. a. N.I. Pirogov
Russian Federation
Russian Federation
Sokolova Natalya A., PhD Med, associate prof. at Dept of Clinical Laboratory Diagnostics of the Faculty of Additional Postgraduate Education.
Moscow
M. I. Savina
Russian National Research Medical University n. a. N.I. Pirogov
Russian Federation
Russian Federation
Savina Marina I., Dr of Bio Sci, prof. at Dept of Clinical Laboratory Diagnostics of the Faculty of Additional Postgraduate Education.
Moscow
O. S. Shokhina
Children’s City Clinic No. 7
Russian Federation
Russian Federation
Shokhina Oksana S., head of Clinical Diagnostic Laboratory.
Moscow
References
1. Lin J., Luo Y., Yao S., Yan M., Li J., Ouyang W., Kuang M. Discovery and correction of spurious low platelet counts due to EDTA-dependent pseudothrombocytopenia. J Clin Lab Anal. 2015; 29 (5): 419–26. https://doi.org/10.1002/jcla.21818
2. Gowland E., Kay H. E., Spillman J. C., Williamson J. R. Agglutination of platelets by a serum factor in the presence of EDTA. J Clin Pathol. 1969; 22 (4): 460–4. http://dx.doi.org/10.1136/jcp.22.4.460
3. Ohashi-Fukuda N., Inokuchi R., Sato H., Nakamura K., Iwagami M., Wada T., Jona M., Hisasue T., Nakajima S., Yahagi N. Poorer Prognosis With Ethylenedi-aminetetraacetic Acid-dependent Pseudothrombocytopenia. Medicine. 2015; 94 (15): 1–4. DOI: 10.1097/MD.0000000000000674.
4. Akbayram S., Dogan M., Akgun C., Caksen H., Oner A. F. EDTA-Dependent Pseudothrombocytopenia in a Child. Сlin Appl Thromb Hemost. 2011; 17 (5): 494–6. https://doi.org/10.1177/1076029610373367
5. Bizzaro N. EDTA-dependent pseudothrombocytopenia: a clinical and epide-miological study of 112 cases, with 10-year follow-up. Am J Hematol. 1995; 50: 103–109. https://doi.org/10.1002/ajh.2830500206
6. Berkman N., Michaeli Y., Or R., Eldor A. EDTA-dependent pseudothrombocytope-nia: a clinical study of 18 patients and a review of the literature. Am J Hematol. 1991; 36 (3): 195–201. DOI: 10.1002/ajh.2830360307.
7. Mannuß S., Schuff-Werner P., Dreißiger K., Kohlschein P. Magnesium Sulfate as an Alternative In Vitro Anticoagulant for the Measurement of Platelet Parameters? Am J Clin Pathol. 2016; 145 (6): 806–14. https://doi.org/10.1093/ajcp/aqw066
8. Sakurai S., Shiojima I., Tanigawa T., Nakahara K. Aminoglycosides prevent and dissociate the aggregation of platelets in patients with EDTA-dependent pseudothrombocytopenia. Br J Haematol. 1997; 99 (4): 817–23. https://doi.org/10.1046/j.1365–2141.1997.4773280.x
Review
For citations:
Sokolova N.A., Savina M.I., Shokhina O.S. EDTA‑dependent pseudothrombocytopenia in child (clinical case report). Medical alphabet. 2021;(13):51-54. (In Russ.) https://doi.org/10.33667/2078-5631-2021-13-51-54
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