

The range of clinical application of Raman fluorescence diagnostics in assessing the state of human tissues in normal and pathological conditions
https://doi.org/10.33667/2078-5631-2025-10-99-108
Abstract
The presented work provides a rationale for the development of an innovative, universal, digital diagnostic complex for express Raman-fluorescence diagnostics. The range and algorithm of its clinical application are presented, providing a sano- and pathogenetic approach to the objectivity and effectiveness of assessing human tissues and organs in health and pathology: diseases and processes of microbial and neoplastic nature (in clinical microbiology, dentistry, obstetrics and gynecology, oncology, gastroenterology, pharmacology). The solution to these issues is based on the methodology of laser digital photometry, basic requirements and principles of modern science and practice, providing controlled treatment of a specific disease in a specific patient. The prospects for the use of the method and equipment are outlined.
Keywords
About the Authors
M. T. AleksandrovRussian Federation
Aleksandrov Mikhail Timofeevich – Doctor of Medical Sciences, Professor, Laureate of the State Prize,
Moscow
V. I. Kukushkin
Russian Federation
Kukushkin V.I. – Candidate of Physical and Mathematical Sciences, Research Associate,
Moscow
A. A. Bashtovoy
Russian Federation
Bashtovoy A.A. – Candidate of Medical Sciences, Associate Professor, Department of Dentistry,
Moscow
References
1. Aleksandrov M.T. Laser clinical biophotometry (theory, experiment, practice). Moscow: Tekhnosfera, 2008:584.
2. Aleksandrov M.T., Afanasyev R.A. et al., Laser fluorescence diagnostics in medicine and biology. Moscow: NPC Spectrolux. 2007:271.
3. Aleksandrov M.T., Zuev V.M., Kukushkin V.I., Karseladze A.I., Ishchenko A.I., Dzhibladze T.A., Metreveli B.G., Khomeriki T.A. Spectral analysis of pelvic tissues in women and it’s clinical value. Oncogynecology. 2013:3:61–67.
4. Aleksandrov M.T., Margaryan E.G. Laser technique application in therapeutic dentistry in clinic (rationale, possibilities, perspectives). Russian Dentistry. 2017: 3:31–36.
5. Aleksandrov M.T., Margaryan E.G. Identification of microorganisms based on the effect giant Raman scattering. Russian Dentistry. 2017: 4:12–19.
6. Aleksandrov M.T., Margaryan E.G. Rationale for the application of surface-enhanced Raman scattering for identification of main pathogens of purulent-inflammatory diseases in maxillofacial area. Dentistry. 2018:1:27–32.
7. Aleksandrov M.T., Kukushkin V.I., Margaryan E.G. Raman fluorescence diagnostics of the state of human tissues in health and pathology and its hardware and software solution. Russian Dental Journal. 2017:5:228.
8. Aleksandrov M.T., Kukushkin V.I., Margaryan E.G., Pashkov E.P., Bagramova G.E. Possibilities and perspectives of raman fluorescence diagnostic application in dentistry. Russian Dental Journal. 2018: 1:4–11.
9. Aleksandrov M.T., Margaryan E.G., Budaychieva Z.S. The application of assessment methods of the psycho-emotional state of (doctor and patient) for gender harmonization of treatment and diagnostic processes in dentistry. Russian Dentistry. 2017:4:42–43.
10. Aleksandrov M.T., Utyuzh A.S., Olesova V.N., Yumashev A.V., Pashkov E.P., Mikhailova M.V., Akhmedov A.N., Dmitrieva E.F., Artemova O.A., Dmitriev A.I., Dzalaeva F.K., Nikolenko D.A. Laser Raman-fluorescence medical technologies in dentistry from experiment to clinic. Moscow: KnigIzdat. 2020: 384.
11. Aleksandrov M.T., Podoynikova M.N., Eganyan D.G. The use of raman-fluorescent medical technologies to assess the effect of physical and chemical factors on the mineralization of hard tooth tissues in normal and noncarious lesions. Russian Dental Journal. 2023:6(27):521–531. doi: 17816/dent508778.
12. Gevorkov GL Complex treatment of patients with phlegmon of the maxillofacial region based on individual selection of an antimicrobial drug using an express method on the laser device «Fluol». Dissertation for the degree of candidate of medical sciences. 2009: 126.
13. Thomas Huser. Nanosensors using Surface-Enhanced Raman Scattering (SERS). Center for Biophotonics Science and Technology. 2007: Feb. 6: EAD289.
14. Kneipp K., Kneipp H., Itzkan I., Dasari R., Feld M.. Surface enhanced Raman scattering and biophysics. Journal of Physics: Condensed Matter. 2002:18: R597–R624.
15. Loschenov V.B, Konov V.I., Prokhorov A.M. Photodynamic Therapy and Fluorescence Diagnostics. Laser Physics, 2000:6:1188–1207.
16. Shcherbo D., Shemiakina I.I., Souslova E.A., Strukova L., Shidlovskiy K.M., Britanova O.V., Zaraisky A.G., Lukyanov K.A., Chudakov D.M., Ryabova A.V., Loschenov V.B., Luker K.E., Schmidt B.T., Luker G.D., Gorodnicheva T.V. Near-infrared fluorescent proteins. Nature Methods. 2010: 10:827-829. doi:10.1038.
17. R. Sheng, F. Nii, T. Cotton, Anal. Chem. 63, 437 (1991).
18. J. Thornton, R. Force, Appl. Spectrosc. 45, 1522 (1991).
19. URL: http://mrl.illinois.edu/sites/default/files/AMC/downloads/PrincetonInstruments_SCT-Spectrograph.pdf
Review
For citations:
Aleksandrov M.T., Kukushkin V.I., Bashtovoy A.A. The range of clinical application of Raman fluorescence diagnostics in assessing the state of human tissues in normal and pathological conditions. Medical alphabet. 2025;(10):99-108. (In Russ.) https://doi.org/10.33667/2078-5631-2025-10-99-108