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Comparative study of microbial contamination of the working surface of different toothbrushs

https://doi.org/10.33667/2078-5631-2025-10-17-18

Abstract

The article presents the results of studying the microbial contamination of manual toothbrushes and PRESIDENT Exlusive toothbrushes before and 1 month after use in 40 patients aged 27±2.3 years without concomitant somatic pathology (the oral cavity is sanitized, the P constant prevails in the DMF index). The toothbrushing time was 3 minutes. When sowing before use, the growth of facultative aerobic gram-positive rods (Bacillus subtilis) and the absence of opportunistic microflora were detected. One month after using PRESIDENT Exlusive toothbrushes, Actinomycetec oralis, Streptococcus Oralis, Lactobacillus salivarius, Str. Vestibularis were detected, which are representatives of the normal microflora. No growth of microorganisms was detected in 5 PRESIDENT Exlusive brushes. One month after using conventional brushes, opportunistic microorganisms Klebsiella oxytoca, Citrobacter freundii, Pseudomonas putida, Candida albicans were found in the cultures along with representatives of normal microflora.

About the Authors

N. V. Tiunova
National Research Lobachevsky State University of Nizhny Novgorod
Russian Federation

Tiunova Natalia Viktorovna – MD, PhD, Associate Professor, Head of the Department
of Clinical Dentistry,

Nizhny Novgorod



E. V. Rumyantseva
National Research Lobachevsky State University of Nizhny Novgorod
Russian Federation

Rumyantseva Ekaterina Vladislavovna – 5th-year student,

Nizhny Novgorod



M. L. Zhdanova
National Research Lobachevsky State University of Nizhny Novgorod
Russian Federation

Zhdanova Maria Lenoidovna - MD, PhD, Associate Professor, Department of Clinical
Dentistry,

Nizhny Novgorod



L. V. Vdovina
Privolzhsky Research Medical University
Russian Federation

Vdovina Lyudmila Valerievna – PhD, Associate Professor of the Department of Propaedeutic Dentistry,

Nizhny Novgorod



I. M. Chuvarkova
Privolzhsky Research Medical University
Russian Federation

Chuvarkova Irina Mikhailovna  – PhD, Associate Professor of the Department of Propaedeutic Dentistry,

Nizhny Novgorod



I. Yu. Shirokova
Privolzhsky Research Medical University
Russian Federation

Shirokova Irina Yuryevna  – Head of the Bacteriological Laboratory, Research Institute
of Preventive Medicine, University Clinic,

Nizhny Novgorod



N. A. Belyanina
Privolzhsky Research Medical University
Russian Federation

Belyanina Natalya Aleksandrovna – Employee of the Bacteriological Laboratory, Research Institute of Preventive Medicine, University Clinic,

Nizhny Novgorod



References

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2. Baluda M.I., Vinnichenko Yu.A., Dmitrieva N.A. Microbiological study of the effectiveness of various methods of toothbrush disinfection. Stomatology. 2012; 1:31–34.

3. Gorodetskaya O.S., Devyatnikova V.G., Trotsky N.D. Features of microbial contamination of toothbrushes in patients with periodontal diseases. Stomatologist. Minsk. 2012;1(4):93–94.

4. Dmitrova A.G., Kulakov A.A., Dmitrieva N.A. Studies of microbial contamination of toothbrushes in children with disabilities. Dentistry for All. 2013;3:52–55.

5. Polyanskaya L.N. et al. Changes in microbial contamination of toothbrushes depending on storage conditions. Dental Journal. 2002;4:29–32.


Review

For citations:


Tiunova N.V., Rumyantseva E.V., Zhdanova M.L., Vdovina L.V., Chuvarkova I.M., Shirokova I.Yu., Belyanina N.A. Comparative study of microbial contamination of the working surface of different toothbrushs. Medical alphabet. 2025;(10):17-18. (In Russ.) https://doi.org/10.33667/2078-5631-2025-10-17-18

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ISSN 2078-5631 (Print)
ISSN 2949-2807 (Online)