Assessment of clinical and laboratory parameters of oral сavity in patients with orthodontic systems

equipment (braces) on the biochemical parameters of oral fluid.

Materials and methods. The study involved patients aged 18 to 22 years who are undergoing orthodontic treatment with a bracket system. Thegroup of examined persons was 25 people. The control group included 15 practically healthy people without braces. All patients underwent dental status assessment (Green – Vermillion hygiene index – simplified IG-Y; PMA inflammation index), laboratory examination of oral fluid before and after controlled oral hygiene. The biochemical analysis included determination of the acid-base balance in the oral fluid using a pH meter, assessment of free radical oxidation by chemiluminogram parameters by biochemiluminescence and by levels of molecular products of lipid peroxidation (diene and triene conjugates, Schiff bases), activity of the enzyme alkaline phosphatase, concentrations of total protein and lactate.

Results and discussions. In patients undergoing orthodontic treatment with a bracket system, an insufficient level of oral hygiene and inflammatory manifestations in the gums of moderate severity were revealed. The level of the acid-base balance of the oral fluid before controlled hygiene was equal to 6.8±0.19, that is, below the range of normal pH values in the oral cavity. After hygiene, the pH of the oral fluid returned to the norm of 7.1±0.2. In patients with braces, activation of free radical oxidation and an increase in lipoperoxidation parameters – triene conjugates and Schiff bases, increased activity of alkaline phosphatase, total protein and lactate levels took place.

Cоnclusion. After hygienic treatment of the oral cavity, the biochemical parameters did not differ statistically significantly from the control data.

1. Domenyuk D.A., Karslieva A.G., Tashueva L.V., Orfanova G.S., Ivancheva E.N., Risovanny S.I. Change of mixed saliva antioxidant sestem of children at the stages of orthodontic treatment using the base matherials. Kuban Scientific Medical Bulletin. 2014;(1):79–84. (In Russ.) https://doi.org/10.25207/1608-6228-2014-1-79-84.

2. Kochurova EV, Kozlov SV. [The diagnostic possibilities of saliva]. Klin Lab Diagn. 2014 Jan;(1):13-5. Russian. PMID: 25069217.

3. Sherstennikova A.K., Kubasova E.D. Clinical and laboratory significance of saliva in the diagnosis of pathological conditions of the human body. Collection of scientific papers based on the results of the scientific and practical conference. 2015; Section No. 48.

4. Cheng Y.S., Rees T., Wright J. A review of research on salivary biomarkers for oral cancer detection. Clin. Transl. Med. 2014; 3(1):3. https://doi.org/10.1186/20011326-3-3

5. Vijayendra P., Vivekananda U., Jyoti T., Smriti P., Gagandeep K. Salivaa diagnostic fluid: a review. International journal of dental and medical research. 2014; 1: 149-153. https://doi.org/10.1034/j.1601-0825.2002.1o834.x

6. Gilmiyarova F.N. Analytical approaches to the study of metabolic parameters in oral fluid. Textbook. M.: Izvestia; 2006.

7. Kuz`mina E.I., Nelyubin A.S., Shchennikova M.K. The use of induced chemiluminescence for the assessment of free radical reactions in biological substrates. [Primenenie indutsirovannoj hemiljuminestsentsii dlja otsenki svobodnoradikal’nyh reaktsij v biologicheskih substratah]. Gor`kiy: Volgo-Vyatskoe izdatel`stvo; 1983. (in Russ.)

8. Volchegorsky I.A., Nalimov A.G., Yarovinsky B.G., Lifshits R.I. Comparison of different approaches to the determination of lipid peroxidation products in heptane-isopropanol extracts of blood. Questions of medical chemistry. 1989. 35; 1:127-3.

9. Daurova A.Z., Lapina N.V., Izhnina E.V., Kochurova E.V., Seferyan K.G., Starchenko T.P. Oral hygiene in patients with fixed orthodontic appliances // Russian Journal of Dentistry. – 2020. – Vol. 24. – N. 2. – P. 104–108. doi: 10.17816/1728-2802-2020-24-2-104-108.

10. Kosyuga SY, Belyakov SA. Povyshenie éffektivnosti ortodonticheskoĭ korrektsii u deteĭ s anomaliiami prikusa i narusheniiami oporno-dvigatel’nogo apparata [Improving the effectiveness of orthodontic correction in children with malocclusion and musculoskeletal disorders]. Stomatologiia (Mosk). 2018;97(5):79–82. Russian. doi: 10.17116/stomat20189705179. PMID: 30346427.

11. Petrova N.P., Sokolovich N.A., Oleinik E.A., Kuzmina D.A., Sverdlova S.V., Efremenko E.I., Saunina A.A. Vliyanie ortodonticheskogo lecheniya na sostoyanie tverdyh tkanej zubov, profilaktika razvitiya oslozhnenij [Influence of orthodontic treatment on the state of dental hard tissues, prevention of complications]. Medical Alliance. 2021; 9(2):56–62