Changes in tissues of taken material at site of thread implantation and IPL therap

Abstract

Material and methods. The article presents the data of a histological study of biopsy material at the site of implantation of the thread, thread and the use of the IPL system on days 14th, 35th. Paraffin sections 5–7 μm thick were made from ready-made paraffin blocks and stained according to the Weigert – Van Gieson method. Using a microscope with a Sony digital photo attachment, 12-megapixel resolution, five photographs were obtained from each histological preparation. Microphotographs using the ImageView, Image Tool 2.00 (GistMorph) programs were used to evaluate the inflammatory response, evaluate the cellular composition, evaluate collagen and elastic fibers, and identify the percentage of collagen type.

Research results. In the biopsy specimen from the site of the implanted thread and IPL technology (Palomar, nozzle 1540, parameters 15, 60, three passes each), when examining histological slides stained according to the Weigert – Van Gieson method, the presence of elastic fibers was revealed, both in the thickness of the dermis and in connective tissue layers of subcutaneous fat. The relative area of elastic fibers in the dermis averaged 13.28 % (min 8.50 %, max 15.50 %). The relative area of elastic fibers in the subcutaneous adipose tissue averaged 2.28 % (min 1.30 %, max 4.80 %). In the biopsy specimen from the site of the implanted thread, the study of histological slides stained according to the Weigert – Van Gieson method revealed the presence of elastic fibers, both in the thickness of the dermis and in the connective tissue layers of the subcutaneous fat. The relative area of elastic fibers in the dermis averaged 13.16 (min 9.70 %, max 15.00 %). The relative area of elastic fibers in the subcutaneous adipose tissue averaged 14.98 % (min 12.20 %, max 16.70 %).

Conclusions. Thus, the study of samples revealed the predominance of type I collagen both in the skin and subcutaneous fat, however, it was less pronounced than with the combined method (implanted thread and IPL technology).