Research of dynamics of monovalent ions in urine at various stages of urolithiasis development in in vivo experiment

Abstract

Introduction. Experimental model of intraperitoneal injection of sodium oxalate is one of the most promising and practical models of oxalate urolithiasis in rodents.

Purpose of the study. To describe the urolithiasis progression in experimental animals with regard to the changes in the ion balance at various stages of pathology formation.

Materials and methods. We treated male ICR (CD‑1) outbred laboratory mice with a single injection of sodium oxalate (NaOx) to perform nephrolithiasis modeling. The ionic composition of urine and blood serum in the control and experimental groups was examined by capillary electrophoresis. The presence of oxalate crystals in the urine sediment and histological data can assess the severity of the pathology.

Results and conclusions. Increase of the level of Na and Ca ions and decrease of the level of K, NH4, Mg ions was observed in 4 hours and 24 hours after intraperitoneal injection of sodium oxalate, that can be regarded as a depression of tubular reabsorption and secretion.