Integrated histological, histochemical and immunohistochemical study of myocardium of rats with experimental hypothyroidism

Experimental study was performed on 66 white male rats Wistar. Experimental model of hypothyroidism by surgery thyroidectomy. The duration of the experiment 45 days. Laboratory animals were taken from the experiment on the 5th, 7th, 14th, 21st, 28th, 35th and 45th day. The control group consisted of 22 rats that surgery thyroidectomy was carried out. In the experiment complied with the international recommendations of the European Convention for the Protection of Vertebrate Animals. For histological examination took pieces of myocardium of the left and right ventricles and the interventricular septum. The pieces were fixed in 10 per cent solution of neutral formalin, preparing histological sections of the standard method. Histological sections were stained with hematoxylin and eosin, pikrofuxin by van Gieson, by toluidine blue, Mallory in the modification of Heidenhain, GOFP (Lilly), laxitives coating on fibrin, spent the SCHICK-reaction. Immunohistochemical studies were performed by peroxidase-antiperoxidase method. Used markers desmin, myogenin, actin sarcomeres. Histological examination of the myocardium revealed vascular disorders: venous plethora, aristrocracy, sludge-phenomenon, diapedetic hemorrhages. In the stroma of myocardium develops diffuse swelling with accumulation of edematous fluid in perivascular and intermuscular spaces. In the myocardium observed effacement of cross-striations, dystrophic changes in cardiomyocytes breach of contracture of myofibrils, pockets of plasmolysis and fragmentation of muscle fibers. In the cytoplasm of cardiomyocytes visible deposits of fibrin. In the stroma of the myocardium showed signs of disorganization of the basic substance of the connective tissue and accumulation of SCHICK-positive substances. Immunohistochemical study, a decrease in positive expression of desmin, myogenin and sarcomere actin with 3 points in the control group to 1 points in the experimental group.

myocardium, cardiomyocytes, interstitial edema, degeneration, plasmolysis, hypothyroidism

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