Determination of carbapenemase genes in clinical isolates using Cepheid Xpert Carba-R
N. I. Gabrielyan,
V. G. Kormilitsyna,
V. K. Zaletaeva,
A. V. Krotevich,
I. A. Miloserdov,
D. A. Saidulaev,
A. S. Dobronogova,
O. V. Kisil https://doi.org/10.33667/2078-5631-2021-18-16-19
Abstract
Detection of carbapenem resistance genes is a critical issue for hospitals due to possible recommendations for infection control and targeted therapy. The Cepheid Xpert instrument, a Carba-R test for the detection and differentiation of five common carbapenemase genes, was tested from September 2020 to February 2021. As part of the approbation, 20 tests were provided. This review presents the results of the approbation of a relatively regular sensitivity study on Siemens WalkAway‑96 plus. Cepheid Xpert Carba-R analysis has been shown to be an accurate and fast tool for detecting colonization by carbapenem-resistant gram-negative bacteria, which can help limit the spread of these organisms in hospitals.
About the Authors
N. I. Gabrielyan
National Medical Research Centre for Transplantology and Artificial Organs n. a. academician V. I. Shumakov
Russian Federation
Russian Federation
Gabrielyan Nina I., DM Sci, head of Dept of Endotoxicosis and Purulent-Septic Complications
Moscow
V. G. Kormilitsyna
National Medical Research Centre for Transplantology and Artificial Organs n. a. academician V. I. Shumakov
Russian Federation
Russian Federation
Kormilitsyna Victoria G., research assistant, laboratory of bacteriology, Dept of Endotoxicosis and Purulent-Septic Infections
Moscow
V. K. Zaletaeva
National Medical Research Centre for Transplantology and Artificial Organs n. a. academician V. I. Shumakov
Russian Federation
Russian Federation
Zaletaeva Viorika K., medical assistant-laboratory, laboratory of bacteriology, Dept of Endotoxicosis and Purulent-Septic Infections
Moscow
A. V. Krotevich
National Medical Research Centre for Transplantology and Artificial Organs n. a. academician V. I. Shumakov
Russian Federation
Russian Federation
Krotevich Alina V., medical assistant-laboratory, laboratory of bacteriology, Dept of Endotoxicosis and Purulent-Septic Infections
Moscow
I. A. Miloserdov
National Medical Research Centre for Transplantology and Artificial Organs n. a. academician V. I. Shumakov
Russian Federation
Russian Federation
Miloserdov Igor A., PhD Med, deputy medical director Saidulaev Dzhabrail A., PhD Med, urologist, surgeon
Moscow
D. A. Saidulaev
National Medical Research Centre for Transplantology and Artificial Organs n. a. academician V. I. Shumakov
Russian Federation
Russian Federation
Saidulaev Dzhabrail A., PhD Med, urologist, surgeon
Moscow
A. S. Dobronogova
Eco-MED-s M’ Co.
Russian Federation
Russian Federation
Dobronogova Anna S., manager
Moscow
O. V. Kisil
Scientific Research Institute for the Research of New Antibiotics n. a. G. F. Gauze
Russian Federation
Russian Federation
Kisil Olga V., PhD Chem, scientific secretary
Moscow
References
1. who.int [Internet]. World Health Organization (WHO). Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics [cited 2020 Aug 12]. Available from: http://www.who.int/medicines/publications/global-priority-list-antibiotic-resistant-bacteria/en/.
2. Nordmann P. Carbapenemase-producing Enterobacteriaceae: overview of a major public health challenge. Med Mal Infect. 2014. Vol. 44. N 2. P. 51–56. DOI: 10.1016/j.medmal.2013.11.007.
3. Chebotar I. V., Bocharova Yu. A., Podoprigora I. V., Shagin D. A. Why Klebsiella pneumoniae becomes a leading opportunistic pathogen. Clinical microbiology and antimicrobial chemotherapy. 2020. No. 1. pp. 4–19. DOI: 10.36488/cmac.
4. Sukhorukova M. V., Eidelshtein M. V., Ivanchik N. V., Skleenova E. Yu., Shaidullina E. R., Azizov I. S., et al. Antibiotic resistance of nosocomial strains of Enterobacterales in hospitals in Russia: results of a multicenter epidemiological study marathon 2015–2016. 2019. No. 2. P. 14–159. DOI: 10.36488/smas.
5. Queenan A. M., Bush K. Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev. 2007. Vol. 20. N 3. P. 440–458. DOI: 10.1128/CMR.00001–07.
6. Oueslati S., Nordmann P., Poirel L. Heterogeneous hydrolytic features for OXA‑48-like β-lactamases. J Antimicrob Chemother. 2015. Vol. 70. N 4. P. 1059–1063. DOI: 10.1093/jac/dku524.
7. Dortet L., Fusaro M., Naas T. Improvement of the Xpert Carba-R Kit for the Detection of Carbapenemase- Producing Enterobacteriaceae. Antimicrob Agents Chemother. 2016. Vol. 60. N 6. P. 3832–3837. DOI: 10.1128/AAC.00517–16.
8. T r a c z e w s k i M . M . , C a r r e t t o E . , C a n t o n R . , Moore N. M. Multicenter evaluation of the Xpert Carba- R assay for detection of carbapenemase genes in Gram-negative isolates. J Clin Microbiol. 2018. Vol. 56. P. e00272–18. DOI: 10.1128/JCM.00272–18.
9. Lledo W., Hernandez M., Lopez E., Molinari O. L., Soto R. Q., Hernandez E. et al. Guidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities. Morbidity and Mortality Weekly Report. 2009. Vol. 58. N. 10. Р. 256–258.
10. Coope C. M., Verlander N. Q., Schneider A., Hopkins S., Welfare W., Johnson A. P., et al. An evaluation of a toolkit for the early detection, management, and control of carbapenemase-producing Enterobacteriaceae: a survey of acute hospital trusts in England. J Hosp Infect. 2018. Vol. 99. N 4. P. 381–389. DOI: 10.1016/j.jhin.2018.03.007.
11. Popov D. A. Comparative characteristics of modern methods for determining the production of carbapenemases. Clinical microbiology and antimicrobial chemotherapy. 2019. No. 2. P. 125–133. DOI: 10.36488/сmac
12. Popov D. A. Comparative characteristics of modern methods for determining the production of carbapenemases. Clinical microbiology and antimicrobial chemotherapy. 2019. No. 2. pp. 125–133. DOI: 10.36488/smas
13. Kost K., Yi J., Rogers B., Jerris R. Comparison of clinical methods for detecting carbapenem- resistant Enterobacteriaceae. Pract Lab Med. 2017. Vol. 18. No. 8. P. 18–25. DOI: 10.1016/j.plabm.2017.03.002.
14. Тato M., Ruiz-Garbajosa P., Traczewski M., Dodgson A. Mc., Ewan A., Humphries R. et al. Multisite evaluation of Cepheid Xpert Carba-R assay for detection of carbapenemase-producing organisms in rectal swabs. J Clin Microbiol. 2016. Vol. 54. P. 1814–1819. DOI: 10.1128/JCM.00341–16.
15. Hoyos-Mallecot Y., Ouzani S., Dortet L., Fortineau N., Naas T. Performance of the Xpert® Carba-R v2 in the daily workflow of a hygiene unit in a country with a low prevalence of carbapenemase-producing Enterobacteriaceae. Int J Antimicrob Agents. 2017. Vol. 49. No. 6. P. 774–777. DOI: 10.1016/j.ijantimicag.2017.01.025.
16. Smith M., Diederen B., Scharringa J., Leversteijn-van Hall M., Fluit A. C., Cohen Stuart J. Rapid and accurate detection of carbapenemase genes in Enterobacteriaceae with the Cepheid Xpert Carba-R assay. J Med Microbiol. 2016. Vol. 65. N 9. P. 951–953. DOI: 10.1099/jmm.0.000310.
17. Ko Y. J., Kim J., Kim H. N., Yoon S. Y., Lim C. S., Lee C. K. Diagnostic performance of the Xpert Carba-R assay for active surveillance of rectal carbapenemase-producing organisms in intensive care unit patients. Antimicrob Resist Infect Control. 2019. Vol. 8. Р. 127. DOI: 10.1186/s13756–019–0579–2
18. Tzouvelekis L. S., Markogiannakis A., Psichogiou M., Tassios P. T., Daikos G. L. Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions. Clin Microbiol Rev. 2012. Vol. 25. Р. 682–707. DOI: 10.1128/CMR.05035–11.
19. Albiger B., Glasner C., Struelens M. J., Grundmann H., Monnet D. L. European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group Carbapenemase-producing Enterobacteriaceae in Europe: assessment by national experts from 38 countries, May 2015. Euro Surveill. 2015. Vol. 20. N 45. P. 30062. DOI: 20. 10.2807/1560–7917.ES.2015.20.45.30062.
Review
For citations:
Gabrielyan N.I., Kormilitsyna V.G., Zaletaeva V.K., Krotevich A.V., Miloserdov I.A., Saidulaev D.A., Dobronogova A.S., Kisil O.V. Determination of carbapenemase genes in clinical isolates using Cepheid Xpert Carba-R. Medical alphabet. 2021;(18):16-19. (In Russ.) https://doi.org/10.33667/2078-5631-2021-18-16-19
Views:
793
Email this article 