Results of protective action of antioxidants on liver in experimental hypothyroidism

Abstract

The work was performed on experimental material. A model of hypothyroidism was obtained in 66 white rats, males of the Wistar strain, by thyroidectomy. Healthy rats aged 8–9 months weighing 250–300 g were selected for the experiment. The operation was performed under general anesthesia. Both thyroid lobes were removed. For histological examination, pieces of gland tissue were taken from both lobes. Pieces were fixed in a 10 % buffered formalin solution for 10 days, then histopreparations were prepared in the standard way. Histological examination revealed diffuse myxedema edema, severe vascular disorders (plethora, stasis, diapedetic hemorrhage), degenerative and destructive changes in hepatocytes, collicative necrosis, plasmolysis, the formation of cavities filled with edematous fluid, atrophy and thinning of the liver beams. When antioxidants α–tocopherol and mexidol are introduced in the postoperative period, stereotypic changes occur: myxedema, vascular disorders, dystrophic and destructive changes, lymphocytic infiltrates, however, these changes occur much later (on the 28th day), are focal in nature, the intensity of edema less pronounced, few lymphocytic infiltrates. When using antioxidants, an increase in reparative processes is observed: the multiplication of binuclear hepatocytes, the proliferation of fibroblasts with the formation of foci of sclerosis.