Study of microbiotain periodontal tissues with periodontitis

The oral cavity is a convenient ecological safe heaven for the opportunistic pathogens, allowing the microorganisms such as Staphylococcus and Candida, to create a biofilm and to develop the mechanisms of antibiotic resistance. His contribution to the pathogenetic process of making viruses, but the role of these bacterial and viral communities remains unclear. In our work we determined the bacterial and viral landscape in periodontal tissues from 82 patients with parodontitis and periodontitis, in a comparison group of 21 people. The bacterial inoculation was performed by classical methods of microbiological research in the Colombian blood agar. Antibiotic resistance genes VIM, NDM, OXA-48, KPC, CTX-M, MecA, TEM, etc. were detected by real-time PCR. Herpes viruses were determined by qualitative PCR. Pathogenic bacteria of the oral cavity Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Porphyromonas endodontalis, Treponema denticola, Tannerella forsythia, Prevotella intermedia, Fusobacterium nucleatum were determined b quantitative PCR. The study of the viral landscape of the content of the parodontum revealed the virus Epstein-Barr with the highest cumulative frequency in the groups with periodontal disease higher than 25 %. Epstein-Barr virus was detected in cultures of Streptococcus pyogenes, Candida albicans, Staphylococcus warneri, Staphylococcus epidermidis, Streptococcus mitis. The genes of antibiotic resistance were detected with low frequency to cephalosporins and carbapenems in the group with generalized periodontitis:MecA - 11,4 %o, NDM - 6,9 %. In the group with chronic periodontitis: MecA - 10,5 %, NDM - 2,6 %, VIM - 2,6 %, OXA-48-2,6 %. Comparison of the data on bacterial and viral load showed a correlation between Epstein-Barr virus with Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans in the pathogenesis of periodontal diseases.

A. actinomycetemcomitans, C. albicans, F. nucleatum, P. gingivalis, T. forsythia, periodontitis, periodontic, clinically significant concentrations, Epstein-Barr virus, A. actinomycetemcomitans, C. albicans, F. nucleatum, P. gingivalis, T. forsythia

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