Biochemical features of alloxan-induced diabetes mellitus

Abstract

The work was carried ouf on experimental material. An experimental model of diabetes mellitus was obtained in 78 white male rats by infraperifoneal administration of alloxane fefrahydrafe150 and 250 mg per 1 kg of body weight. For fhe experiment, healthy rats of adult sex with a mass of250-300 g were selected. Alloxan was administered after a daily starvation, previously determined fhe level of glucose in fhe blood. The rats were kept under optimal conditions, fed according to fhe diet for laboratory animals in accordance with GOST R 50258-92. During fhe experiment, fhe international recommendations of fhe European Convention for fhe Protection of Vertebrates were observed. The material was divided info 2 groups: fhe 1st group consisted of 28 rats administered with alloxan 150 mg per 1 kg, fhe 2nd one consisted of 28 rats administered with alloxan 250 mg per 1 kg. Twenty-two rats were injected with control to which distilled wafer was administered. In laboratory animals of groups I and II, the blood glucose level was increased by 1 day and gradually decreased to normal on fhe 21sf and fhe 35fh days. With fhe introduction of 150 mg per 1 kg, moderate diabetes developed, and with fhe introduction of 250 mg per 1 kg, severe diabetes developed. In laboratory animals of both groups, there was a decrease in body weight, increased wafer intake, and an increase in diuresis. Biochemical indications of blood rats with alloxan-induced diabetes mellitus were characterized by an increase in glycated hemoglobin, cholesterol, low and high density lipoproteins.