Comparative study of the influence of mineral waters of different mineralization “Klyuchi” and “Ust-Kachkinskaya” on the microbiota of the intestinal biotope

Introduction. The qualitative and quantitative uniqueness of the cationic-anionic composition of mineral waters (MW) suggests the presence of specificity of their effects on individual components of the pathogenesis of diseases of the digestive system. In this regard, the study of the bacteriotropic effect of MW on the indigenous microbiota of the intestinal biotope and, accordingly, the assessment of the expediency of their use in dysbiotic conditions is relevant.

Objective. In vitro study of the effect of mineral waters «Klyuchi» and «Ust-Kachkinskaya» on model representatives of the genera Bifidobacterium, Lactobacillus and Escherichia.

Materials and methods. A comparative study of the effect of MW «Klyuchi» and «Ust-Kachkinskaya» on probiotic strains L. plantarum 8P-A3 and B. bifidum 1 was carried out. The level of biomass accumulation, activity of acid formation and utilization of the carbohydrate component during cultivation on a «hungry» nutrient medium was evaluated. A bioluminescence test was used to determine the effect of MW on the physiological state of cells of the E. coli lum+ indicator strain. A comparative study of the kinetics of its luminescence after rehydration from the lyophilized state with various solvents has been carried out.

Results. Both types of MW had a stimulating effect on lactobacilli. The increase in biomass of the tested strain on a «hungry» nutrient medium in both cases was close in magnitude and significantly exceeded the control level. The acid formation activity of lactobacilli was also enhanced by both MW with a more pronounced effect in the «Ust-Kachkinskaya» water. At the same time, MW «Klyuchi» had a significant stimulating effect on the accumulation of biomass and acid formation of bifidobacteria in the absence of these effects in MW “Ust-Kachkinskaya”. The presence of the stimulating effect of MW was confirmed by an increase in the consumption of the carbohydrate component of the nutrient medium. The result of the bioluminescence test also demonstrated a more pronounced stimulating effect of MW «Klyuchi» on the restoration of intracellular metabolic processes after rehydration of the indicator strain E. coli lum+. The level of its luminescence in this sample exceeded by more than two times the luminescence intensity of the samples from MW «Ust-Kachkinskaya» and the control with saline solution.

Conclusion. The positive effect of the studied MW on model representatives of the indigenous microbiota was revealed with a more pronounced stimulating effect of drinking low-mineralized MW “Klyuchi” in comparison with the medium-mineralized MW «Ust-Kachkinskaya».

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