Immunohistochemical and histological diagnosis of acute coronary syndrome

Abstract

The work is performed on autopsy material. Histological and immunohistochemical study of the myocardium 105 deaths from acute coronary syndrome. Histological study was performed according to standard methods used staining with hematoxylin and eosin, pikrofuksin by van Gieson, by toluidine blue, Mallory. Immunohistochemical study was conducted peroxidase-antiperoxidase method using markers desmin, actin sarcomere (SA), myogenin, VWF (factor VIII). Autopsy material was divided into two groups: the first group consisted of 30 (29.6 %) cases with ischemic dystrophy of myocardium, the second one consisted of 75 (71.4 %) cases with myocardial infarction. Of the 105 cases, 63.8 % were men, 36.2 % were women. In ischemic dystrophy of myocardium occurs granular dystrophy of cardiomyocytes, decreases the content of glycogen disappears transverse striations, observed fragmentation of the fibers, focal leukocyte infiltrates. Immunohistochemical study decreasing expression of desmin, actin sarcomeres, myogenin, factor VIII. In the second group monitoring is determined by the focus of coagulation necrosis. Immunohistochemical study of the expression of the above markers negative.