medalphabetМедицинский алфавитMedical alphabet2078-56312949-2807ООО «Альфмед»10.33667/2078-5631-2024-16-58-64medalphabet-3841Research ArticleСтатьиИспользование подсластителей и сахарозаменителей в пищевой промышленности. Сладкие белки как новая альтернативаThe use of sweeteners and sugar substitutes in the food industry. Sweet proteins as a new alternativehttps://orcid.org/0000-0001-6114-564XМаевИ. И.MaevI. V.

Маев Игорь Вениаминович, акад. РАН, д. м. н., проф., зав. кафедрой пропедевтики внутренних болезней и гастроэнтерологии лечебного факультета НОИ клинической медицины им. Н. А. Семашко»

Москва

Maev Igor V., DM Sci (habil.), academician of the Russian Academy of Sciences, head of Dept of Propaedeutics of Internal Diseases and Gastroenterology at Scientific and Educational Institute of Clinical Medicine named after N. A. Semashko

Moscow

igormaev@rambler.ruhttps://orcid.org/0000-0002-7923-9916ЗаборовскийА. В.ZaborovskyA. V.

Заборовский Андрей Владимирович, д. м. н., доцент, зав. кафедрой фармакологии лечебного факультета НОИ клинической медицины им. Н. А. Семашко

Москва

Zaborovskiy Andrey V., DM Sci (habil.), associate professor, head of Dept of Pharmacology at Scientific and Educational Institute of Clinical Medicine named after N. A. Semashko

Moscow

zaborovsky_av@msmsu.ruhttps://orcid.org/0000-0002-0254-0516ЦарегородцевС. В.TsaregorodtsevS. V.

Царегородцев Сергей Вадимович, преп. кафедры фармакологии лечебного факультета НОИ клинической медицины им. Н. А. Семашко

Москва

Tsaregorodtsev Sergei V., lecturer at Dept of Pharmacology at Scientific and Educational Institute of Clinical Medicine named after N. A. Semashko

Moscow

sergiotsar@yandex.ruhttps://orcid.org/0000-0001-8901-9557ЮнинаД. В.YuninaD. V.

Юнина Дина Владимировна, преподаватель кафедры фармакологии лечебного факультета НОИ клинической медицины им. Н. А. Семашко

Москва

Yunina Dina V., lecturer at Dept of Pharmacology at Scientific and Educational Institute of Clinical Medicine named after N. A. Semashko

Moscow

yunina@msmsu.ruhttps://orcid.org/0000-0003-1506-2565КолосовЮ. А.KolosovYu. A.

Колосов Юрий Антольевич, к. м. н., доцент, заместитель директора по учебной работе НОИ фармации им К. М. Лакина

Москва

Kolosov Yuri A., PhD Med, associate professor, deputy director for Educational Affairs of K. M. Lakin Scientific and Educational Institute of Pharmacy

Moscow

tronk79@gmail.comhttps://orcid.org/0009-0003-8598-022XСтаровойтоваМ. Ю.StarovoytovaM. Yu.

Старовойтова Мария Юрьевна, студентка, лечебный факультет

Москва

Starovoytova Maria Y., student at Faculty of Medicine.

Moscow

masha.starovojtova2000@yandex.ruhttps://orcid.org/0000-0001-8006-698XТараринаЛ. А.TararinaL. A.

Тарарина Лариса Анатольевна, старший преподаватель кафедры фармакологии лечебного факультетата НОИ клинической медицины им. Н. А. Семашко

Москва

Tararina Larisa A., senior lecturer at Dept of Pharmacology at Scientific and Educational Institute of Clinical Medicine named after N. A. Semashko

Moscow

79104906528@yandex.ruФГБОУ ВО «Российский университет медицины» Минздрава РоссииРоссияFederal State Budgetary Educational Institution of Higher Education “Russian University of Medicine” of Ministry of Health of RussiaRussian FederationЮнина Дина Владимировна, преподаватель кафедры фармакологии лечебного факультета НОИ клинической медицины им. Н. А. СемашкоРоссияFederal State Budgetary Educational Institution of Higher Education “Russian University of Medicine” of Ministry of Health of RussiaRussian Federation202405092024016Диетология и нутрициология58‑64Copyright © Маев И.И., Заборовский А.В., Царегородцев С.В., Юнина Д.В., Колосов Ю.А., Старовойтова М.Ю., Тарарина Л.А., 20242024Маев И.И., Заборовский А.В., Царегородцев С.В., Юнина Д.В., Колосов Ю.А., Старовойтова М.Ю., Тарарина Л.А.Maev I.V., Zaborovsky A.V., Tsaregorodtsev S.V., Yunina D.V., Kolosov Y.A., Starovoytova M.Y., Tararina L.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.med-alphabet.com/jour/article/view/3841

В данном обзоре рассматриваются последствия чрезмерного употребления сладких продуктов на здоровье человека и исследуется потенциал сахарозаменителей и интенсивных подсластителей, включая сладкие белки, в качестве их более здоровых альтернатив. Анализ статистических данных о распространенности диабета и ожирения выявляет риски для здоровья, связанные с высоким потреблением сахара. Произведен обширный обзор свойств различных сахарозаменителей и подсластителей с акцентом на их гликемический индекс и уровень сладости. Особое внимание уделено сладким белкам, таким как монеллин и браззеин, их структуре и биологическим эффектам. Сладкие белки обладают значительно высоким индексом сладости и могут служить альтернативой традиционному сахару в производстве пищевых продуктов. Однако их применение в пищевой или фармацевтической промышленности ограничено. Исследование подчеркивает потенциал сахарозаменителей и подсластителей, особенно сладких белков, для разработки продуктов питания за счет снижения содержания сахара. Тем не менее требуются дальнейшие исследования безопасности сладких белков и понимания их долгосрочных эффектов на здоровье человека.

This review examines the consequences of excessive consumption of sweet products on human health and explores the potential of sugar substitutes and intensive sweeteners, including sweet proteins, as their healthier alternatives. Analyzing statistical data on the prevalence of diabetes and obesity, the study highlights the health risks associated with high sugar intake. An extensive review of the properties of various sugar substitutes and sweeteners has been conducted, focusing on their glycemic index and sweetness level. Special attention is given to sweet proteins, such as monellin and brazzein, their structure, and biological effects. Sweet proteins have a significantly high sweetness index and can serve as an alternative to traditional sugar in food production. However, their application in the food or pharmaceutical industry is limited. The study emphasizes the potential of sugar substitutes and sweeteners, especially sweet proteins, in developing food products by reducing sugar content. Nonetheless, further research is required to ensure the safety of sweet proteins and understand their long-term effects on human health.

сахарный диабетожирениесахарозаменителиподсластителисладкие белкимонеллинбраззеинdiabetesobesitysugar substitutessweetenerssweet proteinsmonellinbrazzeinReferencesДедов И. И., Шестакова М. В., Викулова О. К. и др. Эпидемиологические характеристики сахарного диабета в Российской Федерации: клинико-статистический анализ по данным Федерального регистра сахарного диабета на 01.01.2021. Сахарный диабет. 2021; 24 (3): 204–221.Dedov I. I., Shestakova M. V., Vikulova O. K. et al. Epidemiological characteristics of diabetes mellitus in the Russian Federation: clinical and statistical analysis according to the Federal diabetes register data of 01.01.2021. Diabetes mellitus. 2021; 24 (3): 204–221. (In Russ.). https:.doi.org/10.14341/DM12759Xiaoda S., Yuting Y., Li L. et al. Design and development of a high temperature stable sweet protein base on monellin. Process Biochemistry. 2020; 89: 29–36.Xiaoda S., Yuting Y., Li L. et al. Design and development of a high temperature stable sweet protein base on monellin. Process Biochemistry. 2020; 89: 29–36.Kayode R. M.O., Abiodun O. A., Akeem S. A., Oyeneye H. O. Influence of partial substitution of sugar with serendipity berry (dioscoreophyllum cumminsii) extract on the quality attributes and shelf-life of wheat bread. J. Microbiol Biotech Food Sci / Kayode et al. 2019; 9(1): 115–120.Kayode R. M.O., Abiodun O. A., Akeem S. A., Oyeneye H. O. Influence of partial substitution of sugar with serendipity berry (dioscoreophyllum cumminsii) extract on the quality attributes and shelf-life of wheat bread. J. Microbiol Biotech Food Sci / Kayode et al. 2019; 9(1): 115–120.Spadaccini R., Crescenzi O., Tancredi T. et al. Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin. Journal of Molecular Biology. 2001; 305: 505–514.Spadaccini R., Crescenzi O., Tancredi T. et al. Solution structure of a sweet protein: NMR study of MNEI, a single chain monellin. Journal of Molecular Biology. 2001; 305: 505–514.Ming D., Hellekant G. Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett. 1994; 355(1):106–8. DOI: 10.1016/0014–5793(94)01184–2Ming D., Hellekant G. Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett. 1994; 355(1):106–8. DOI: 10.1016/0014–5793(94)01184–2Izawa H., Ota M., Kohmura M., Ariyoshi Y. Synthesis and characterization of the sweet protein brazzein. Biopolymers. 1996; 39 (1): 95–101. DOI: 10.1002/(SICI)1097–0282(199607)39:1<95: AID-BIP10>3.0.CO;2-BIzawa H., Ota M., Kohmura M., Ariyoshi Y. Synthesis and characterization of the sweet protein brazzein. Biopolymers. 1996; 39 (1): 95–101. DOI: 10.1002/(SICI)1097–0282(199607)39:1<95: AID-BIP10>3.0.CO;2-BPfeiffer J. F., Boulton R. B., Noble A. C. Modelling the sweetness response using time-intensity data. Food Quality & Preference. 2000; 11: 129–134.Pfeiffer J. F., Boulton R. B., Noble A. C. Modelling the sweetness response using time-intensity data. Food Quality & Preference. 2000; 11: 129–134.Hellekant G., Danilova V. Brazzein a Small, Sweet Protein: Discovery and Physiological Overview. Chem. Senses. 2005; 30 (suppl 1): i88–i89. DOI: 10.1093/chemse/bjh127Hellekant G., Danilova V. Brazzein a Small, Sweet Protein: Discovery and Physiological Overview. Chem. Senses. 2005; 30 (suppl 1): i88–i89. DOI: 10.1093/chemse/bjh127Ming D., Markley J. L., Hellekant G. Quantification of Cysteinyl Sulfhydryl Residues in Peptides and Proteins by ESIMS or MALDIMS. BioTechniques. 1995; 18 (5): 808–10.Ming D., Markley J. L., Hellekant G. Quantification of Cysteinyl Sulfhydryl Residues in Peptides and Proteins by ESIMS or MALDIMS. BioTechniques. 1995; 18 (5): 808–10.Jin Z., Danilova V., Assadi-Porter F.M. et al. Critical regions for the sweetness of brazzein. FEBS Lett. 2003. https: doi.org/10.1016/S 0014–5793(03)00383–1Jin Z., Danilova V., Assadi-Porter F.M. et al. Critical regions for the sweetness of brazzein. FEBS Lett. 2003. https: doi.org/10.1016/S 0014–5793(03)00383–1Assadi-Porter F.M., Aceti D. J., Cheng H., Markley J. L. Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin. Arch. Biochem. Biophys. 2000, 376 (2): 252-–258. DOI: 10.1006/abbi.2000.1725Assadi-Porter F.M., Aceti D. J., Cheng H., Markley J. L. Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin. Arch. Biochem. Biophys. 2000, 376 (2): 252-–258. DOI: 10.1006/abbi.2000.1725Kinnamon S. C. Taste receptor signaling- from tongues to lungs. Acta Physiol (Oxf). 2012; 204 (2): 158–68.Kinnamon S. C. Taste receptor signaling- from tongues to lungs. Acta Physiol (Oxf). 2012; 204 (2): 158–68.Cui M., Jiang P., Maillet E. et al. The heterodimeric sweet tase receptor has multiple potential ligand binding sites. Curr Pharm Des. 2006; 12 (35): 4591–600.Cui M., Jiang P., Maillet E. et al. The heterodimeric sweet tase receptor has multiple potential ligand binding sites. Curr Pharm Des. 2006; 12 (35): 4591–600.Lazutkaite G., Solda A., Lossow K. et al. Amina acid sensing in hypothalamic tanycytes via umami taste receptors. Mol. Metab. 2017; 6(11):1480–1492. DOI: 10.1016/j.molmet.2017.08.015Lazutkaite G., Solda A., Lossow K. et al. Amina acid sensing in hypothalamic tanycytes via umami taste receptors. Mol. Metab. 2017; 6(11):1480–1492. DOI: 10.1016/j.molmet.2017.08.015Chen A. N.Y., Hellyer S. D., Trinh P. N.H. et al. Identification of monellin as the first naturally-derived proteinaceous allosteric agonist of metabotropic glutamate receptor 5. Basic Clin Pharmacol Toxicol. 2020; 126(6):104–115. DOI: 10.1111/bcpt.13239Chen A. N.Y., Hellyer S. D., Trinh P. N.H. et al. Identification of monellin as the first naturally-derived proteinaceous allosteric agonist of metabotropic glutamate receptor 5. Basic Clin Pharmacol Toxicol. 2020; 126(6):104–115. DOI: 10.1111/bcpt.13239Cancellere R. Metabolic effects of the sweet protein MNEI as a sweetener in drinking water. A pilot study of a high fat dietary regimen in a rodent model. Nutrients. 2019; 11(11): 2643.Cancellere R. Metabolic effects of the sweet protein MNEI as a sweetener in drinking water. A pilot study of a high fat dietary regimen in a rodent model. Nutrients. 2019; 11(11): 2643.Chung J. H., Kong J. N., Choi H. E., Kong K. H. Antioxidant, anti-inflammatory, and anti-allergic activities of the sweet-tasting protein brazzein. Food Chemistry. 2018; 267: 163–169.Chung J. H., Kong J. N., Choi H. E., Kong K. H. Antioxidant, anti-inflammatory, and anti-allergic activities of the sweet-tasting protein brazzein. Food Chemistry. 2018; 267: 163–169.Suez J., Korem T., Zeevi D. et al. Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature. 2014; 514:181–186. DOI: 10.1038/nature13793Suez J., Korem T., Zeevi D. et al. Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature. 2014; 514:181–186. DOI: 10.1038/nature13793Pfeffer M., Ziesenitz S. C., Siebert G. Acesulfame K, cyclamate and saccharin inhibit the anaerobic fermentation of glucose by intestinal bacteria. Z. Ernahrungswiss. 1985; 24 (4): 231–5. DOI: 10.1007/BF02023668Pfeffer M., Ziesenitz S. C., Siebert G. Acesulfame K, cyclamate and saccharin inhibit the anaerobic fermentation of glucose by intestinal bacteria. Z. Ernahrungswiss. 1985; 24 (4): 231–5. DOI: 10.1007/BF02023668Suez J., Korem T., Zilberman-Schapira G. et al. Non-caloric artificial sweeteners and the microbiome: findings and challenges. Gut Microbes. 2015; 6: 149–55.Suez J., Korem T., Zilberman-Schapira G. et al. Non-caloric artificial sweeteners and the microbiome: findings and challenges. Gut Microbes. 2015; 6: 149–55.Rubenstein L., Robinson J., Seguin R. A. et al. Diet drink consumption and the risk of cardiovascular events: a report from the Women’s Health Initiative. J. Gen. Intern. Med. 2015; 30 (4): 462–8. DOI: 10.1007/s11606–014–3098–0Rubenstein L., Robinson J., Seguin R. A. et al. Diet drink consumption and the risk of cardiovascular events: a report from the Women’s Health Initiative. J. Gen. Intern. Med. 2015; 30 (4): 462–8. DOI: 10.1007/s11606–014–3098–0de Koning L., Malik V. S., Kellogg M. D. et al. Sweetened Beverage Consumption, Incident Coronary Heart Disease, and Biomarkers of Risk in Men. Circulation. 2012; 125: 1735–1741. https: doi.org/10.1161/CIRCULATIONAHA.111.067017de Koning L., Malik V. S., Kellogg M. D. et al. Sweetened Beverage Consumption, Incident Coronary Heart Disease, and Biomarkers of Risk in Men. Circulation. 2012; 125: 1735–1741. https: doi.org/10.1161/CIRCULATIONAHA.111.067017Duffey K. J., Steffen L. M., Van Horn L. et al. Dietary patterns matter: diet beverages and cardiometabolic risks in the longitudinal Coronary Artery Risk Development in Young Adults (CARDIA) Study. Am J. Clin. Nutr. 2012; 95 (4): 909–915. DOI: 10.3945/ajcn.111.026682Duffey K. J., Steffen L. M., Van Horn L. et al. Dietary patterns matter: diet beverages and cardiometabolic risks in the longitudinal Coronary Artery Risk Development in Young Adults (CARDIA) Study. Am J. Clin. Nutr. 2012; 95 (4): 909–915. DOI: 10.3945/ajcn.111.026682Gardener H. R.T., Markert M., Wright C. B. et al. Diet soft drink consumption is associated with an increased risk of vascular events in the northern manhattan study. J. Gen. Intern. Med. 2012; 27:1120–1126. DOI: 10.1007/s11606–011–1968–2Gardener H. R.T., Markert M., Wright C. B. et al. Diet soft drink consumption is associated with an increased risk of vascular events in the northern manhattan study. J. Gen. Intern. Med. 2012; 27:1120–1126. DOI: 10.1007/s11606–011–1968–2Swithers S. E. Artificial sweeteners produce the counterintuitive effect of inducing metabolic derangements. Trends Endocrinol Metab. 2013; 24 (9): 431–41. DOI: 10.1016/j.tem.2013.05.005Swithers S. E. Artificial sweeteners produce the counterintuitive effect of inducing metabolic derangements. Trends Endocrinol Metab. 2013; 24 (9): 431–41. DOI: 10.1016/j.tem.2013.05.005Schiano C., Grimaldi V., Scognamiglio M. et al. Soft drinks and sweeteners intake: Possible contribution to the development of metabolic syndrome and cardiovascular diseases. Beneficial or detrimental action of alternative sweeteners? Food. Res. Int. 2021; 142: 110220. DOI: 10.1016/j.foodres.2021.110220Schiano C., Grimaldi V., Scognamiglio M. et al. Soft drinks and sweeteners intake: Possible contribution to the development of metabolic syndrome and cardiovascular diseases. Beneficial or detrimental action of alternative sweeteners? Food. Res. Int. 2021; 142: 110220. DOI: 10.1016/j.foodres.2021.110220Chazelas E., Debras C., Srour B. et al. Sugary Drinks, Artificially-Sweetened Beverages, and Cardiovascular Disease in the NutriNet-Santé Cohort. JACC Journals. 2020; 76 (18): 2175–2177.Chazelas E., Debras C., Srour B. et al. Sugary Drinks, Artificially-Sweetened Beverages, and Cardiovascular Disease in the NutriNet-Santé Cohort. JACC Journals. 2020; 76 (18): 2175–2177.Kramers P. G.N. The mutagenicity of saccharine. Mutation research. Reviews I Genetic Toxicology. 1975; 32 (1): 81–92.Kramers P. G.N. The mutagenicity of saccharine. Mutation research. Reviews I Genetic Toxicology. 1975; 32 (1): 81–92.Assadi-Porter F.M., Maillet E. L., Radek J. T. et al. Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R 2-T1R 3 human sweet receptor. Journal of molecular biology. 2010; 398 (4): 584–599.Assadi-Porter F.M., Maillet E. L., Radek J. T. et al. Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R 2-T1R 3 human sweet receptor. Journal of molecular biology. 2010; 398 (4): 584–599.

The authors declare that there are no conflicts of interest present.