medalphabetМедицинский алфавитMedical alphabet2078-56312949-2807ООО «Альфмед»10.33667/2078-5631-2022-19-44-48medalphabet-2791Research ArticleСтатьиВозможности и ограничения методов подсчета тромбоцитовPossibilities and limitations of platelet counting methodshttps://orcid.org/0000-0003-4464-6367ЦветковаТ. Г.TsvetkovaT. G.

Татьяна Геннадьевна Цветкова, врач КЛД, зав. лабораторией

ФГБУ «Всероссийский центр экстренной и радиационной медицины имени А. М. Никифорова»

лаборатория гематологических и общеклинических методов исследования

Санкт-Петербург

Tatyana G. Tsvetkova, doctor of KLD, head of the Laboratory

The Nikiforov Russian Center of Emergency and Radiation Medicine

Laboratory of Hematological and General Clinical Research Methods

Saint Petersburg

tsvetkofa@yandex.ruhttps://orcid.org/0000-0002-5338-0866КалашниковаА. А.KalashnikovaA. A.

Анастасия Андреевна Калашникова,  к. б. н., с. н. с

ФГБУ «Всероссийский центр экстренной и радиационной медицины имени А. М. Никифорова»

НИЦ

НИО лабораторной диагностики

Санкт-Петербург

Anastasia A. Kalashnikova, PhD Med, senior researcher

The Nikiforov Russian Center of Emergency and Radiation Medicine

National Research Centre

Research Dept of Laboratory Diagnostics

Saint Petersburg

petkova_nas@mail.ruМЧС РоссииРоссияNRCERM EMERCOM of RussiaRussian Federation202216102022019Современная лаборатория (2)4448Copyright © Цветкова Т.Г., Калашникова А.А., 20222022Цветкова Т.Г., Калашникова А.А.Tsvetkova T.G., Kalashnikova A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.med-alphabet.com/jour/article/view/2791

   В настоящее время использование гематологических анализаторов позволяет осуществить быстрый и точный подсчет количества тромбоцитов в подавляющем большинстве образцов крови. В некоторых случаях автоматическое определение этого показателя может быть некорректным, что связано с ограничениями используемых методов. Контроль результатов автоматического подсчета тромбоцитов необходим при получении патологических значений и осуществляется при микроскопическом исследовании мазка периферической крови. В статье изложены способы и ограничения автоматического определения количества тромбоцитов, возможные причины ошибочного подсчета и пути коррекции результата. Представлены морфологические описания наиболее часто встречающихся артефактов, влияющих на правильное определение этого показателя. Освещены современные методические подходы к решению задачи корректного подсчета тромбоцитов при ЭДТА-зависимой псевдотромбоцитопении.

   Currently, the use of hematological analyzers makes it possible to quickly and accurately count the number of platelets in the vast majority of blood samples. In some cases, the automatic determination of this indicator may be incorrect, due to the limitations of the methods used. Monitoring the results of automatic platelet count is necessary when obtaining pathological values and is carried out by microscopic examination of a peripheral blood smear. The article describes the methods and limitations of automatic determination of the number of platelets, possible causes of erroneous counting and ways to correct the result. Morphological descriptions of the most common artifacts that affect the correct definition of this indicator are presented. Modern methodological approaches to solving the problem of correct platelet counting in EDTA-dependent pseudothrombocytopenia are highlighted.

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The authors declare that there are no conflicts of interest present.